The scaffolded activities will guide your students in the understanding of main idea and how to write their own main idea statement based on a passage. This lesson will help students learn how to write a main idea statement and how to differentiate the main idea from topic, a detail from the text, and theme. Practice #4: Students write their own short story that includes a clear theme and write 4 multiple choice questions based on their short story.Ĭheck In: 10 question assessment that covers vocabulary, understanding the concepts with 2 short passages, and applying the concepts to an excerpt from Growing Up by Gary Soto.įormatted PDF versions of the activities and check inĭetermining the main idea is a foundational skill for all students. Practice #3: Students read a short fable and answer 4 multiple choice questions about the main idea, objective summary, and theme. Practice #2: Students read a short passage and complete an objective summary graphic organizer. Practice #1: Students determine if statements are examples of main idea or theme. They are perfect for providing targeted practice and review in small chunks. Provide your students with focused practice on main idea, objective summary, and theme with these engaging and rigorous bell ringer activities. Standards Bell Ringer Series: Main Idea, Objective Summary, Theme Students will analyze what will change in their favorite movie or story if one of the story elements was different create an original story that follows the plot diagram and turn it into a picture book write a one-act play based on someone or an event in history. Students will read Hou Yi and the Ten Suns and complete graphic organizers based on the story elements read an excerpt from Piecing Me Together and use the templates provided to create a set of Instagram stories for the main character read an excerpt from Refugee and compare the story elements between the different chapters. Students will work with understanding vocabulary identify the story elements in the Pixar short, Wind and use the templates provided to create an Instagram page that teaches the meaning of the story elements. The engaging and creative activities range from building foundational skills to applying the concepts. Differentiate and increase interest for your students with this leveled choice board for story elements.
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This will help other buyers choose a reliable seller and help a good seller get more orders. Redeem your CD key and confirm the order completion.Select an offer with the game you want and contact a seller.Our support service, which is available 24/7, will assist you in resolving any issues you may have. The rating system, which makes it easy to choose the most reliable seller.Market prices, which are achieved due to competition between sellers.Instant delivery: at any time, you can choose a seller who is currently online.Why buy Tom Clancy’s Ghost Recon CD Keys on FunPay Until then, the payment will be put on hold by the security system and will only be transferred to the seller after you redeem the CD key and confirm the order completion. All deals on our marketplace are protected because the payment is transferred to the seller’s account only after they give you the CD key. The Tom Clancy’s Ghost Recon series of games will brighten up your evenings, and FunPay will make sure that the purchase of a CD key brings only positive emotions. Tom Clancys Ghost Recon Breakpoint is all about your choice, your way to have fun. To navigate the game world more quickly, players have access not only to cars, motorcycles, and boats but also helicopters. You can find them in the hiding places of enemy gangs, so you’ll be encouraged to explore another point on the map not only because of tasks but also because of the opportunity to obtain new weapons. You’ll need good weapons in Tom Clancy’s Ghost Recon games, you can modify and customize your weapons, changing or adding details and attachments. You’ll need to uncover information about the enemy leader and eliminate them this is the main mission objective. During the game, you’ll come across various notes and clues that lead to other locations or connections within the enemy gang. The plot revolves around a squad of elite soldiers who are sent on reconnaissance missions, and you need to locate enemy gang members. If you purchased a game from the Epic Games Store website, it’ll be available for download in the Library section of your Epic Games Launcher. While tasks or missions provide specific objectives or locations on the map, you are also free to follow them or explore what you see in the distance. You dont need a product key to activate games that you purchase directly from the Epic Games Store. The story of the game encourages players to explore the world and discover interesting places. Army Special Forces soldiers who were sent on a top-secret mission to eliminate dangerous criminals. You’ll play as one of “the Ghosts”, a squad of U.S. Ghost Recon: Breakpoint is exclusive to Epic, a possible Matrix sequel, and a Suicide Squad 2 casting.Subscribe to IGN for more. Here, you can also drive a car, encountering patrols, bases, and roadblocks and clearing out enemies. The gameplay of Ghost Recon is similar to that of Far Cry, another series of games from Ubisoft. You can play in co-op with friends or explore the open world alone. the game has to be activated via uplay and be there in your library as well as on epic games. Question Ive got Wildlands on Steam but then Breakpoint isnt available on steam. If you like shooting and fast-paced action, FunPay offers you to buy Tom Clancy’s Ghost Recon CD keys without overpaying. The official Subreddit for Ubisofts Original Tom Clancys Ghost Recon. everytime you run this game again after you close it then you need to press the green play button again from steam library to run it with dx11.Tom Clancy’s Ghost Recon is a series of military tactical shooter video games inspired by the American novelist Tom Clancy. the 2nd option is to go to your steam library again and choose breakpoint and right click to breakpoint properties and choose the launch option there, switch it out to dx11 instead of vulkan. To run this game with dx11 you need to go to your steam library, choose breakpoint then you can press the green play button and it will ask you to use which version of the game either dx11 or vulkan. Has anyone experienced the same? Any ideas how to fix this or force the game to launch with dx 11? dx11 can't be launch with that "-dx11" parameter for this game. A year after launch, Breakpoint finally delivers most of what fans expected from Ghost Recon although some flaws are too intrinsic to the game design to ever be fixed. I've tried setting -dx11 in launch options, tried uninstalling ubi connect and forcing it to reacquire, still launching with the vulkan executable as seen in task manager. Benchmark shows the app running vulkan, not directx 11. Originally posted by deciBelle:I've started with a clean install and before even touching anything, switched to launching with directx 11 in the game's properties -> launch option. This loan’s first payment would be divided as $37.50 toward interest, $162.50 toward paying down principal.Subtract the first month’s interest charge from the fixed payment amount to see what portion of the first month’s payment will be applied to the loan’s principal: $162.50.Divide that number by 12 to get the first month’s interest charge: $37.50.Multiply the total amount of the loan by the interest rate: 450.What would your amortization schedule look like? Here’s how to start your amortization chart, beginning with the first month: Your interest rate is 3%, and the loan term is 5 years. Let’s say you have a car loan of $15,000. To calculate basic amortization and create an amortization schedule, you need the following information: Make better decisions backed by data and insights Learn More How to Create an Amortization Schedule For now, let’s focus on amortization and amortization schedules as they apply to loan repayment. There is another financial situation in which you might come across amortization: bookkeeping and taxes for a small business or freelance gig, wherein the cost of an asset is spread out across the lifetime of the asset. Interest costs will be a higher portion of your monthly payment at the beginning of the loan because it is a percentage of your outstanding principal as more of your monthly payments are applied to principal, the interest costs shrink. At its most basic, amortization is paying off a loan over a fixed period of time (the loan term) by making fixed payments that are applied toward both loan principal (the original amount borrowed) and interest (the charge for taking out the loan, a percentage of the principal).įor example, if you are paying a mortgage, car loan, or student loan with a fixed interest rate, your monthly payment will remain the same over the lifetime of your loan, but the amount of each payment that goes toward principal and interest will change. 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Convert Apple Worldwide Developer Relations Certification Authority certificate to pem format: openssl x509 -in AppleWWDRCA.cer -inform DER -out AppleWWDRCA.pem -outform PEMĤ. pem format: openssl x509 -inform der -in distribution.cer -out distribution.pemģ. Openssl genrsa -out distribution.key 2048Ģ. Last and final thing you’ll need to do in this step is convert the Mac Developer Installer certificate & Mac Developer Application certificate to a p12 format.įollow this process twice for each certificate: Now copy the Issuer ID & KEY ID, download the API Key and save all three of them for later. Select the Keys tab and on the side menu Key Type select App Store Connect API.Ĭlick the blue + button beside the Active title.Įnter a Name and Access for the key and click Generate: Head back over to Program resources in the main Account dashboard and select Users and Access under App Store Connect (shown above). Now that we have the certificates, it’s time to get the notarization stuff.Ģ. 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The adventurous Greeks of Miletus, in Asia Minor, formed several colonies around the Black Sea coast during the 6th and 7th centuries BCE in order to exploit its resources for trade with the wider Greek world. Of course, some Total War games are bound to be better than others for a bevy of reasons, but chances are that fans will get their money's worth from any title they pick out in a series known for upping the ante every single time. The Cimmerian Bosporus has been fought over for its resources and strategic importance for millennia. The massive scale of the simulations that take place in the overworld coupled with a focus on proper tactics in each battle makes things a blast in every way, with players loving how the series never ceases to amaze them with a combination of brilliant gameplay coupled with memorable moments that will stick with players no matter which game they decide to play. As a playable faction, Rome benefits economically from its excellence in metalwork, enjoys enhanced military development, and can exploit the masses in order to maintain public. These monolithic formations are supported by the Roman cavalry, or equites. Athenai Boiotain League Korinthos Sparta Total War Rome II - All Desert Kingdoms Black. Total War: Rome II Factions Units Auxiliary Corps Units in Custom Battle Buildings Technologies Household Regions Mercenaries Total War: Rome II: MODs Radious Total War Mod Divide et Impera Total War: Rome II: ICONs BullGods Unit Icons Normals Rome IIUnits Icons. Rome Carthage Syracuse Lusitani Arevaci Wrath of Sparta. Rome Arverni Nervii Suebi Hannibal at the Gates. All intros for all factions in the 'Imperator Augustus' campaign. 2.7K 172K views 2 years ago fightme rome2shorts totalwarrome2gameplay I Made a total war Rome 2 Faction Tier List and Ranked all the factions in there I will be explaining the. Updated Februby Ritwik Mitra: Total War is one of the greatest strategy video game franchises of all time. The oldest and most experienced warriors the bronze-armoured triarii hold the rear. Total War Rome II - All Imperator Augustus Faction Intros. Great starting position, good mix of hellenic defensive units with decent attacking barbarian infantry. I like how they have the armored Indian elephants, Silver Shields, good archers, and a ton of satrapies to start out with. From the turn-based empire simulation to the real-time wars that require the best tactics possible for players to succeed, it's easy to see why Total War games have such a dedicated and passionate fanbase who aren't afraid to speak their minds. My favorite Greek faction in Rome 2 is the Seleucids. Regardless of which Total War game players get into for the first time, there's no denying that these titles just have something special going for them that really resonates with fans when things just click. This collection contains 15 parts, each containing about 20 albums. That's why we have compiled a comprehensive collection of Buckethead's discography in high-quality FLAC format, which you can download from the link below. However, finding and downloading his albums can be a challenge, as some of them are rare, out of print, or only available on his website. If you are a fan of Buckethead, or curious about his music, you might be interested in his discography, which spans over two decades and covers a wide spectrum of musical styles and moods. He has collaborated with many artists, such as Guns N' Roses, Les Claypool, Bootsy Collins, Serj Tankian, and Viggo Mortensen. He is known for his signature look of wearing a white mask and a KFC bucket on his head, as well as his virtuosic and expressive playing style. Regardless, with much of the emotion left out of the equation for me, ‘Soothsayer (Dedicated To Aunt Suzie)’ remains one of the most mesmerising songs I’ve ever heard, and one of the most technically amazing guitar tracks ever written.Buckethead Discography - 1991-2012 15īuckethead is a prolific and enigmatic guitarist who has released over 300 albums in various genres, ranging from experimental rock to ambient to funk to metal. While I obviously cannot relate precisely to the inspiration behind this song, I can understand the sentiment that goes along with dedicating a song to someone, and can only image how emotional it must have been. Sadly, Buckethead’s aunt Suzie passed away the following year, with the guitarist composing the track ‘ Sail On Soothsayer (In Memory Of Aunt Susie 1932-2007)‘ for his Decoding The Tomb Of Bansheebot album. Described as the woman who gifted him his first guitar, the track is almost equal parts a tribute to this woman, and a way for him to showcase his stunning abilities as one of the most amazing guitarists alive today. After hearing it a couple of times, it became a song that I couldn’t ignore, it’s melodic and technical proficiency was absolutely mesmerising to a guitarist like myself, and I couldn’t help but wonder what possessed him to be able to write such a stunning piece of music like this.Īs I soon discovered, like how Colma was written for his sick mother, ‘Soothsayer’ was written for his sick aunt Suzie. While I listened to tracks like this, I much preferred the more acoustic or ambient works of albums such as Electric Tears or Colma.Įventually, ‘Soothsayer’ became one of those tracks that I couldn’t resist. While I went and bought copies of Colma, Kaleidoscalp, and the collaborative effort, Enter The Chicken, I felt as though he was a musician who needed to stay something of a secret, lest his brilliance be corrupted by the masses.Īs a result, I wasn’t exactly a fan of albums such as 2006’s Crime Slunk Scene, which saw tracks like ‘Soothsayer (Dedicated To Aunt Suzie)’ become frequent favourites among fans on online communities. While I loved his early work, his more polished tunes from the turn of the millennium really began to resonate with me. At this point in time, his discography was manageable, with only 18 albums to his name (as of November 2018, he’s now got 306 albums), and I was able to jump in and experience everything. However, it was in late 2006 when his song ‘Jordan’ came my way thanks to Guitar Hero II.Īn immensely fast track, ‘ Jordan‘ was like nothing I’d ever heard before, and I needed to know more about this stunning musician. Quite possibly, it was in relation to his rather short stint as a guitarist for Guns N’ Roses that ended rather abruptly. I had heard of Buckethead in passing somewhere around 2005 or so. Variability in western blotting occurs most often with unequal protein concentrations, inconsistent sample loading onto the gel, and irregularities during transfer – all normal, human realities of the process. Quantitative western blotting requires careful interpretation, particularly around non-uniformities. With a quantitative western blot, researchers can gain valuable insight into relative protein expression. Quantitative Western Blotting Requires Normalization So, what does a “publication-ready” western blot mean today to the top journals in the field, and what tips, tools, and products exist to help researchers get there? This comprehensive guide will help steer you through the process. Their updated guidelines for immunoblotting images come alongside guardrails against data manipulation, intentional or inadvertent, in a growing age of generative AI and accessible image editing software. Researchers can now reliably measure relative changes in protein expression and abundance.īut with this shift towards quantitative understanding comes a movement by major journals, funding agencies, and professional scientific societies to uphold the highest standards of data integrity for publication. In recent years, however, improved western blot technology and technique have allowed an exciting quantitative element to emerge. In its early days, the assay delivered information in a strict binary of ‘yes’ or ‘no’ – protein present or absent. Western blotting is a powerful tool in the sense that it can tease out the needle – a single protein of interest – in a proverbial haystack sample containing hundreds of proteins.įor more than 40 years, the western blot or immunoblot has been a workhorse of the wet lab. More Resources for Western Blot Reporting Journal-Specific Western Blot Publication GuidelinesĪmerican Association of Cancer Research (AACR) Multiplexing can capture up to four proteins in a single blot for more meaningful and representative experiments, a must for western blot publication.įor today’s top journals, total protein normalization and high-quality images are the keys to western blot publication It also produces publishable figures which indicate exactly the quantification areas which is extremely important for a transparent measurement.Fluorescence detection multiplexing achieves more data from your sample with iBright. I will try to get in contact with the authors and see if there might be a possibility to link it as plugin into ImageJ (e.g. It is freely available but not completely open source with access to the source code. The design of a quantitative western blot experimentģ.) Since I first tried to code a plugin which would combine the necessary functions for ImageJ, I stumbled over an existing and extremely good tool (also Java) with all the things you would need to run such an analysis (including calibration).Western Blotting Inaccuracies with Unverified Antibodies: Need for a Western Blotting Minimal Reporting Standard (WBMRS).Evaluating Strategies to Normalise Biological Replicates of Western Blot Data.A Defined Methodology for Reliable Quantification of Western Blot Data (Here you see the analysis methos indicated).Quantifying Western blots: Pitfalls of densitometry.So, here a few recommendations and very helpful links (since this is not my invention):ġ.) have a look at the following video seminar from Aldrin Gomes as an introduction to the problem on the experimental side:Ģ.) Here a few publications which help in getting a better understanding before the actial image analysis So, even while often suggested, drawing boxes around a band is not the proper way! 2: The ►Analyze ►Gel tools and the ►Analyze ►Calibrate… function provide theoretically everything what you would need but a clear guideline is still missing. That’s why I posted it here!Įverything I have found in the web so far was mostly going a in the wrong direction leading to non-reliable measurements! 1: Many students try to find a guideline for Western blot “quantification” online while using ImageJ. And I think the topic is important in the aspect of reliability and reproducibility in science. So the following topic is not directly linked to ImageJ necessarily but many of this community might still be interested in. Since once in a while the topic semi-quantitative Western blot analysis pops up and many people in biological sciences who do Western blots or gels are into that, I wanted to share some information on that. Once you have selected your new characters, click on ‘Copy’.Ĥ Now you need to paste the characters you’ve copied in to Silhouette Studio. If you have more than one character you want to change, then follow the same process, and that character will appear in the same box. Now choose the characters you want to replace by clicking on the character and pressing the select button, this will then appear in the ‘Characters to copy’ box. If you click on one of the characters a little box will appear with a larger example of the ‘character’ This box will not disappear and will stay on the screen. To access the special characters, tick the ‘Advanced View’ Box, in the Group by drop down box choose ‘Unicode Subrange’, a little box will pop up on the right, select the ‘Private Use Characters’ option. (Make a note of the font size for use further on in this guide)īefore we go any further we need to access the ‘Character Map’, follow the next steps to open the ‘Character Map’ 3 Using the search function on your PC type in Character Map, once found click on it to open the character map. Now using the text style box on the right, select the font you want to use (it has to be one you know is PUA encoded and has different glyphs) I’m using the Hedgehog font from the Hungry JPEG – which is currently only $1 and comes with a Full Commercial License – Bargain!Īdjust the Text Size to whatever size you wish, right click on the font and select the ‘Convert to Path’ option. For the purpose of this tutorial I’m using ‘addicted to fonts’ – because, who isn’t? :-) Using the Text Tool, type out the phrase or quote you want to use. The font will be installed and will now be accessible via Silhouette Studio.Ģ Open Silhouette Studio as you normally would. The following preview will pop up on the screen, click install. Click on either the TTF or OTF file, either one will work. Once you have downloaded and extracted the font files, navigate to where you extracted the files to. Unzip the file by right clicking and selecting ‘Extract all’, choose a file you want the font to be ‘extracted’ too.įor further guidance, check out my tutorial on how to unzip files here. Once you have downloaded, navigate to the downloads folder on your PC. In the following tutorial, I’ve put together a quick guide on how to use PUA encoded fonts in Silhouette Studio ġ First off download the font from the download link, the download link will be wherever you have purchased your font from, you may have even had an email with the download link. You would normally need fancy software to access extra Glyphs, Swirls, Flourishes and Font Characters, but with PUA encoded fonts you can access these within your Silhouette Studio Software on your windows PC. You would need to check if any of the other editions can do this with the text panel.Fonts, Fonts, everywhere! Fonts are perfect for designing your own quotes and cutting with vinyl to put on a Wooden Plaque, Mugs, Glass Blocks etc. I also have the business edition so I am not sure if this is only available with this edition. I am sure there is other ways of getting to the glyph menu but for me this is easier and I am not searching my computer for the character map. It helps if you color the letters and make your comment or text bigger to get a good feel of how it will look when your done. After you pick your first letter I just double click on it and it comes up on my design area, then I can add the next letter that I want if for some reason I don't like that choice you just use the backspace and try a different one. The one nice thing with using this is the zoom slider at the bottom of the panel which helps seeing what you might like to use and another thing I like is some fonts don't offer any glyphs and when that happens you can see it at a glance that there is no fancy options so you can go to the next. Click the G and this will have your fonts in a drop down menu, select the one you want to work with it will then show you all of the letters and or characters. After you open the program you would open the "text style" panel, then the tabs offer the A for text style, G for your Glyphs or the ABC for spelling. You can access them thru the Silhouette Studio. There is another way to get to the glyph characters. |
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